Sheep model for osteoporosis: sustainability and biomechanical relevance of low turnover osteoporosis induced by hypothalamic-pituitary disconnection.

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Autor/in:
Erscheinungsjahr:
2013
Medientyp:
Text
Schlagworte:
  • Animals
  • Female
  • Disease Models, Animal
  • Ovariectomy
  • Sheep
  • Biomechanics/physiology
  • Osteogenesis/physiology
  • Osteoblasts/metabolism/pathology
  • Bone and Bones/*metabolism/pathology
  • Bone Resorption/physiopathology
  • Hypothalamus/*physiology/*surgery
  • Osteoclasts/metabolism/pathology
  • Osteoporosis/*metabolism
  • Pituitary Gland/*physiology/*surgery
  • Animals
  • Female
  • Disease Models, Animal
  • Ovariectomy
  • Sheep
  • Biomechanics/physiology
  • Osteogenesis/physiology
  • Osteoblasts/metabolism/pathology
  • Bone and Bones/*metabolism/pathology
  • Bone Resorption/physiopathology
  • Hypothalamus/*physiology/*surgery
  • Osteoclasts/metabolism/pathology
  • Osteoporosis/*metabolism
  • Pituitary Gland/*physiology/*surgery
Beschreibung:
  • Hypothalamo-pituitary disconnection (HPD) leads to low bone turnover and osteoporosis in sheep. To determine the sustainability of bone loss and its biomechanical relevance, we studied HPD-sheep 24 months after surgery (HPD + OVX-24) in comparison to untreated control (Control), ovariectomized sheep (OVX), and sheep 12 months after HPD (HPD + OVX-12). We performed histomorphometric, HR-pQCT, and qBEI analyses, as well as biomechanical testing of all ewes studied. Twenty-four months after HPD, histomorphometric analyses of the iliac crest showed a significant reduction of BV/TV by 60% in comparison to Control. Cortical thickness of the femora measured by HR-pQCT did not change between 12 and 24 months after HPD but remained decreased by 30%. These structural changes were caused by a persisting depression of osteoblast and osteoclast cellular activity. Biomechanical testing of the femora showed a significant reduction of bending strength, whereas calcium content and distribution was found to be unchanged. In conclusion, HPD surgery leads to a persisting low turnover status with negative turnover balance in sheep followed by dramatic cortical and trabecular bone loss with consequent biomechanical impairment.

Lizenz:
  • info:eu-repo/semantics/restrictedAccess
Quellsystem:
Forschungsinformationssystem des UKE

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oai:pure.atira.dk:publications/09a62a26-ed72-4cf8-9e93-2d2c07fdc8ab