Cloning, expression, purification and preliminary X-ray analysis of a putative metagenome-derived lipase

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Autor/in:
Erscheinungsjahr:
2012
Medientyp:
Text
Schlagworte:
  • Amino Acid Sequence
  • Bacteria/enzymology
  • Cloning, Molecular
  • Conserved Sequence
  • Crystallography, X-Ray
  • Gene Expression
  • Lipase/chemistry
  • Metagenome
  • Molecular Sequence Data
  • Sequence Alignment
Beschreibung:
  • LipS is a novel thermostable putative lipase that was isolated from a metagenomic library using functional screening methods. The corresponding gene shows high similarity to that encoding a putative but uncharacterized esterase from Symbiobacterium thermophilum IAM14863 (99% nucleotide-sequence similarity). Two different constructs of the recombinant lipase were crystallized. Crystals belonging to space group P42212 diffracted X-ray radiation to 2.8 Å resolution and crystals belonging to space group P4 diffracted to 2.0 Å resolution. The most probable content of their asymmetric units were two molecules (P42212) and four or five molecules (P4), respectively. © 2012 International Union of Crystallography. All rights reserved.
Lizenz:
  • info:eu-repo/semantics/closedAccess
Quellsystem:
Forschungsinformationssystem der UHH
Interne Metadaten
Quelldatensatz
oai:www.edit.fis.uni-hamburg.de:publications/3958615b-f7c5-4d7a-b475-8a96b1b4c513