Isolation, purification, crystallization and preliminary crystallographic studies of a chitinase from Crocus vernus

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Autor/in:
Erscheinungsjahr:
2011
Medientyp:
Text
Schlagworte:
  • Chitinase
  • Pathogens
  • Chitinase gene
  • Arabidopsis
  • Plants
  • Genes
  • Chitinase
  • Pathogens
  • Chitinase gene
  • Arabidopsis
  • Plants
  • Genes
Beschreibung:
  • A chitinase has been isolated and purified from Crocus vernus corms. N - terminal amino-acid sequence analysis of the approximately 30 kDa protein showed 33% identity to narbonin, a seed protein from Vicia narbonensis L. The C. vernus chitinase was crystallized by the hanging-drop vapour-diffusion method using PEG 8000 as the main precipitant. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 172.3, b = 37.1, c = 126.4 Å, β = 127° and two molecules per asymmetric unit. Diffraction data were collected to a resolution of 2.1 Å. © 2011 International Union of Crystallography All rights reserved.
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  • info:eu-repo/semantics/closedAccess
Quellsystem:
Forschungsinformationssystem der UHH
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Quelldatensatz
oai:www.edit.fis.uni-hamburg.de:publications/83ba4918-1c94-4420-934c-ca5ba9160cc3