The German Bight is a hot-spot of eutrophication in the North Sea due to nitrate loads discharged by several large rivers into this semi-isolated embayment. River nitrate loads have a distinctly higher N-15/N-14 ratio than nitrate in open North Sea waters, and to trace the sphere of river influence we analysed stable isotope signatures of water column nitrate in the area on a grid of stations in winter and early spring 2007. Overall spatial patterns of N-15/N-14 and O-18/O-16 in nitrate reflect the predominant influence of nitrate discharged by the Rhine and Elbe rivers on the German Bight nitrate pool. On a smaller scale, however, and in offshore stations, nitrate assimilation of an incipient phytoplankton bloom is indicated by parallel enrichment of N-15 and O-18 in nitrate. Intriguingly, the enrichment ratio of O-18(NO3) to N-15(NO3) is 1.6:1, thus differing from the ratio of 1:1 associated with uptake by marine phytoplankton. This suggests that nitrate isotopic composition is not solely affected by phytoplankton assimilation, but that a substantial portion of nitrate in the outer regions of the German Bight is derived from nitrification, despite low ambient temperatures. Moreover, the data identify remineralisation and nitrification of particulate N in sediments as important sources of dissolved inorganic nitrogen to the German Bight water column, and underscore the role of sediments in recharging water column nutrient inventories.