A bioassay has been carried out to determine the uptake of selected heavy metals from freshwater sediments by Ankistrodesmus bibraianus Korshikov and Entermorpha intestinalis Link. An apparatus was used in which a 0.45 µm pore diameter membrane separated the algae and sediments. When used with freshwater and sediments, this method indirectly demonstrated biological uptake from resuspended sediments. The amounts of metals accumulated in the test algae were compared to chemical associations of metals in sediments, but there were no clear relationships with geochemical phases. The observed relationships between growth and metal content of the algae indicated, based on biomass, that Cu reduced algal growth during the 96-h test period. The distributions of metals in water, sediments, and algae were influenced by salinity, and marked differences were apparent among the six elements studied.