Bacteria differently regulate mRNA abundance to specifically respond to various stresses
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- Erscheinungsjahr:
- 2016
- Medientyp:
- Text
- Schlagwort:
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- Journal Article
- Beschreibung:
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Environmental stress is detrimental to cell viability and requires an adequate reprogramming of cellular activities to maximize cell survival. We present a global analysis of the response of Escherichia coli to acute heat and osmotic stress. We combine deep sequencing of total mRNA and ribosome-protected fragments to provide a genome-wide map of the stress response at transcriptional and translational levels. For each type of stress, we observe a unique subset of genes that shape the stress-specific response. Upon temperature upshift, mRNAs with reduced folding stability up- and downstream of the start codon, and thus with more accessible initiation regions, are translationally favoured. Conversely, osmotic upshift causes a global reduction of highly translated transcripts with high copy numbers, allowing reallocation of translation resources to not degraded and newly synthesized mRNAs.
- Lizenz:
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- info:eu-repo/semantics/closedAccess
- Quellsystem:
- Forschungsinformationssystem der UHH
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- Quelldatensatz
- oai:www.edit.fis.uni-hamburg.de:publications/471ca82f-a12b-4a02-b767-6075b821a40a