Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky
Erscheinungsjahr:
2011
Medientyp:
Text
Schlagworte:
Sternzelle
Cholecystokinin
Gastrin
Kollagensynthese
stellatecells
pancreas
cholecystokinine
collagensynthesis
610 Medizin, Gesundheit
44.61 Innere Medizin
44.87 Gastroenterologie
ddc:610
Beschreibung:
The gastrointestinal hormone cholecystokinin (CCK) can induce acute pancreatitis in rodents through its action on acinar cells. Treatment with CCK, in combination with other agents, represents the most commonly used model to induce experimental chronic pancreatitis. Pancreatic stellate cells (PSC) are responsible for pancreatic fibrosis and therefore play a predominant role in the genesis of chronic pancreatitis. However, it is not known whether PSC express CCK-receptors. Using real-time PCR techniques, we demonstrate that CCK1- and CCK2-receptors are expressed on rat PSC. Interestingly both CCK and gastrin significantly induced type I collagen synthesis. Moreover, both inhibit proliferation. These effects are comparable to TGF-beta- stimulated PSC. Furthermore, the natural agonists CCK and gastrin induce activation of pro-fibrogenic pathways Akt, ERK and Src. Using specific CCK1- and CCK2-receptor inhibitors, we found that Akt activation is mainly mediated by CCK2R. Akt activation by CCK and gastrin could be inhibited by PI3K inhibitor wortmannin. Activation of ERK and downstream target Elk-1 could be inhibited by MEK-inhibitor U0126. These data suggest that CCK and gastrin have a direct activating effect on PSC, are able to induce collagen synthesis in these cells and therefore appear to be important regulators of pancreatic fibrogenesis. Furthermore, similar to TGF-beta both CCK and gastrin inhibit proliferation in PSC.