Tryptophan 13C nuclear-spin polarization generated by intraprotein electron transfer in a LOV2 domain of the blue-light receptor phototropin

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Autor/in:
Erscheinungsjahr:
2009
Medientyp:
Text
Schlagworte:
  • Light
  • Optogenetics
  • Light oxygen
  • Riboflavin
  • Chemically induced dynamic nuclear polarization (CIDNP)
  • Nuclear-spin polarization
  • Electron transfer
  • Flavin radical
  • Radical-pair mechanism
  • Tryptophan radical
  • Light
  • Optogenetics
  • Light oxygen
  • Riboflavin
Beschreibung:
  • 13C-NMR experiments were performed on photo-excited fully and partially 13c-labelled LOV2 domains of the blue-light receptor phototropin. In the present paper, we report on nuclear-spin polarized tryptophan resonances that are generated by light-induced intraprotein electron transfer to the FMN cofactor. The spectra are discussed with respect to earlier data obtained from 13C-NMR experiments on unlabelled LOV2 domains that have been reconstituted with FMN 13C isotopologues. © The Authors Journal compilation © 2009 Biochemical Society.
Lizenz:
  • info:eu-repo/semantics/closedAccess
Quellsystem:
Forschungsinformationssystem der UHH
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oai:www.edit.fis.uni-hamburg.de:publications/e85ae11a-aab5-4dae-b3ac-8cc79b70ad9e