This paper aims to analyse the maturity of the Brazilian entrepreneurial ecosystem based on the opinions and insights from business incubator professionals. To this end, a survey was performed with 74 professionals who published at least one paper related to this topic in Brazil. The maturity analysis was based on a theoretical model with variables to be measured for the incubators in six major constructs: support, government, finance, human capital, culture and market. Data was analysed through structural equation modelling, which allowed for a statistic validation of the model. With this validation, it was possible to analyse the maturity notes assigned to each variable and conclude that the Brazilian entrepreneurial ecosystem has an intermediate level of maturity, with some possible improvements. The originality of this study lies both in the assessment carried out and in the use of this particular statistical technique for this purpose.