Three-dimensional reconstruction of the giant mimivirus particle with an X-ray free-electron laser

Link:
Autor/in:
Erscheinungsjahr:
2015
Medientyp:
Text
Schlagworte:
  • Free electron lasers
  • Ionization
  • Two-photon double
  • Harmonic Generations
  • Ultrashort Pulses
  • Laser Pulses
  • Free electron lasers
  • Ionization
  • Two-photon double
  • Harmonic Generations
  • Ultrashort Pulses
  • Laser Pulses
Beschreibung:
  • We present a proof-of-concept three-dimensional reconstruction of the giant mimivirus particle from experimentally measured diffraction patterns from an x-ray free-electron laser. Three-dimensional imaging requires the assembly of many two-dimensional patterns into an internally consistent Fourier volume. Since each particle is randomly oriented when exposed to the x-ray pulse, relative orientations have to be retrieved from the diffraction data alone. We achieve this with a modified version of the expand, maximize and compress algorithm and validate our result using new methods.
Lizenz:
  • info:eu-repo/semantics/restrictedAccess
Quellsystem:
Forschungsinformationssystem der UHH

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Quelldatensatz
oai:www.edit.fis.uni-hamburg.de:publications/e27586ba-9383-47c5-b2f8-3d9102fdcd34