A combination of experiments and molecular dynamics simulation for the investigation of the ion-exchange adsorption of biological macromolecules

Link:
Autor/in:
Verlag/Körperschaft:
Hamburg University of Technology
Erscheinungsjahr:
2013
Medientyp:
Text
Schlagworte:
  • Hemoglobin
  • Ion-exchange adsorption
  • MD simulation
  • Serum albumin
  • SMA model
  • 540: Chemie
  • 660: Technische Chemie
Beschreibung:
  • To get a comprehensive understanding of the adsorption of macromolecules onto ion-exchange adsorbent, the single component ion-exchange adsorptions of serum albumin and hemoglobin onto Q Sepharose FF were investigated with both adsorption equilibrium experiments and molecular dynamics (MD) simulations. Both of the investigations show that serum albumin adsorbs stronger than hemoglobin in the condition studied. The steric mass-action model was introduced to describe the adsorption equilibrium of both proteins, whose parameters were also studied with MD simulations. The results of MD simulations are qualitatively consistent with those of experiments. © 2013 Elsevier B.V.
Beziehungen:
DOI 10.1016/B978-0-444-63234-0.50005-1
Quellsystem:
TUHH Open Research
Interne Metadaten
Quelldatensatz
oai:tore.tuhh.de:11420/7649