Crystalline Biohybrid Materials Based on Protein Cages

Link:
Autor/in:
Beteiligte Personen:
  • Ueno, Takafumi
  • Lim, Sierin
  • Xia, Kelin
Verlag/Körperschaft:
Humana Press Inc.
Erscheinungsjahr:
2023
Medientyp:
Text
Schlagworte:
  • Ferritin protein cage
  • Highly ordered nanoparticle superlattices
  • Metal oxide nanoparticles
  • Protein crystallization
  • Protein design
  • Crystallization
  • Ferritins
  • Metal Nanoparticles
  • Nanostructures
  • Oxides
  • cerium oxide nanoparticle
  • ferritin
  • iron oxide nanoparticle
  • protein
  • metal nanoparticle
  • nanomaterial
  • oxide
  • amino acid sequence
  • asepsis
  • cation exchange
  • cell culture
  • crystal structure
  • fast protein liquid chromatography
  • ion exchange
  • protein purification
  • protein structure
  • protein synthesis
  • room temperature
  • waste disposal
  • X ray crystallography
  • crystallization
Beschreibung:
  • Highly ordered superstructures of nanomaterials can be synthesized using protein cages as templates for the assembly of inorganic nanoparticles. Here, we describe in detail the creation of these biohybrid materials. The approach involves computational redesign of ferritin cages, followed by recombinant protein production and purification of the new variants. Metal oxide nanoparticles are synthesized inside the surface-charged variants. The composites are assembled using protein crystallization to yield highly ordered superlattices, which are characterized, for example, with small angle X-ray scattering. This protocol provides a detailed and comprehensive account on our newly established strategy for the synthesis of crystalline biohybrid materials. © 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Lizenz:
  • info:eu-repo/semantics/closedAccess
Quellsystem:
Forschungsinformationssystem der UHH
Interne Metadaten
Quelldatensatz
oai:www.edit.fis.uni-hamburg.de:publications/ab04763b-38bd-471b-b778-7a667cbb722b