Synthesis of nucleoside di- and triphosphates and dinucleoside polyphosphates with cyclosal-nucleotides

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Autor/in:
Erscheinungsjahr:
2009
Medientyp:
Text
Schlagworte:
  • Nucleosides
  • Nucleotides
  • Cap analogs
  • Oligonucleotides
  • DNA
  • Nucleosides
  • Nucleotides
  • Cap analogs
  • Oligonucleotides
  • DNA
Beschreibung:
  • A new and efficient method for the synthesis of nucleoside di- and triphosphates as well as dinucleoside polyphosphates (NpnN') is described. 5-Acceptor-substituted (5-nitro and 5-chloro) cycloSal-nucleotides are used as starting material that were reacted with a variety of phosphate nucleophiles as pyrophosphate or nucleotides to the corresponding products in short times and very good yields. After consumption of the starting cycloSal-phosphate triester, first the protecting groups were cleaved and finally the products were isolated after RP-column chromatography. Examples are shown for all five pyrimidine and purine bases found in natural nucleosides as well as one non-natural pyrimidine base to prove that the method can be applied generally. © 2009 American Chemical Society.
Lizenz:
  • info:eu-repo/semantics/closedAccess
Quellsystem:
Forschungsinformationssystem der UHH
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Quelldatensatz
oai:www.edit.fis.uni-hamburg.de:publications/490408cc-d660-4380-aaf6-03f6bc7142ac