A method to determine the throughput speed of a pore

A method (100) to determine the throughput speed v of at least one pore (1), comprising the steps of: • feeding (110), by means of a driving force F, a filiform calibration element (2) through the pore (1), said calibration element (2) having a plurality of markers (21) spaced apart by known distances (22) and configured to produce an interaction event (3) that transmits a signal away from the pore (1) upon interaction with the pore (1); • detecting (120) a plurality of interaction events (3); and determining (130) a time interval Δt between successive interaction events (3), and/or a frequency of interaction events (3). A filiform calibration element (2), configured to pass through a pore (1) of at most 5 jam diameter and comprising a plurality of light sources, configured to absorb excitation light of a first wavelength λ1 and to emit light of a second wavelength λ2 in response to said excitation light, as markers (21). A liquid solution (20) as a tool for determining the throughput speed v of at least one pore (1), comprising a solvent (24) and a plurality of filiform calibration elements (2). (Figure 1)