A chemo-enzymatic approach for site-specific modification of the RNA cap

Link:

https://doi.org/10.1002/anie.201302874

Autor/in:

Erscheinungsjahr:

2013

Medientyp:

Text

Schlagworte:

AdoMet analogues
click chemistry
mRNA
trimethylguanosinesynthase

Beschreibung:

Capped and gowned: A two-step approach can be used to site-specifically modify the 5′-cap of eukaryotic mRNAs. First, a trimethylguanosinesynthase variant recognizes the m7G cap structure and introduces bioorthogonal groups using S-adenosyl-L-methionine-based cosubstrates. Then, the enzymatically introduced reporter groups are further modified by thiol-ene or CuAAC click chemistry (see scheme). Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Lizenz:

info:eu-repo/semantics/closedAccess

Quellsystem:

Forschungsinformationssystem der UHH

Interne Metadaten

Quelldatensatz: oai:www.edit.fis.uni-hamburg.de:publications/9902c8f0-c85a-46aa-8525-92a79d022564