Impact of the NK Cell Receptor LIR-1 (ILT-2/CD85j/LILRB1) on Cytotoxicity against Multiple Myeloma
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Einfluss des NK-Zell Rezeptors LIR-1 (ILT-2/CD85j/LILRB1) auf die Zytotoxizität gegen das Multiple Myelom
Staats- und Universitätsbibliothek Hamburg Carl von Ossietzky
Erscheinungsjahr:
2012
Medientyp:
Text
Schlagworte:
NK Zelle
LIR-1
CD85j
LILRB1
Multiples Myelom
NK cell
LIR-1
CD85j
LILRB1
Multiple Myeloma
610 Medizin, Gesundheit
44.45 Immunologie
44.81 Onkologie
44.86 Hämatologie
Plasmozytom
ddc:610
Plasmozytom
Beschreibung:
The role of different receptors in natural-killer- (NK-) cell-mediated cytotoxicity against multiple myeloma (MM) cells is unknown. The present work investigated if an enhancement of NK-cell-mediated cytotoxicity against MM could be reached by blocking of the inhibitory leukocyte immunoglobulin-like receptor 1 (LIR-1). The investigations revealed high levels of LIR-1 expression not only on the NK cell line NK-92, but also on myeloma cells (MOLP-8, RPMI8226) as well as on a lymphoblastoid cell line (LBCL; IM-9). Subsequent cytotoxicity assays were designed to show the isolated effects of LIR-1 blocking on either the effector or the tumor side to rule out receptor-receptor interactions. Although NK-92 was shown to be capable of myeloma cell lysis, inhibition of LIR-1 on NK-92 did not enhance cytotoxicity. Targeting the receptor on MM and LBCL did not also alter NK-92-mediated lysis. The conclusion was that LIR-1 alone does not directly influence NK-cell-mediated cytotoxicity against myeloma. This work provides the investigation of the inhibitory capability of LIR-1 in NK-92-mediated cytotoxicity against MM and the functional evaluation of LIR-1 on MM and LBCL.