The last decades have seen dramatic changes in the hydrography and biogeochemistry of the Mediterranean Sea. The complex bathymetry, highly variable spatial and temporal scales of atmospheric forcing and internal processes contribute to generate complex and unsteady circulation patterns and significant variability in biogeochemical systems. Part of this variability can be influenced by anthropogenic contributions. Consequently, it is necessary to document details and to understand trends in place to better relate the observed processes and to possibly predict the consequences of these changes. In this context we report on data from an oceanographic cruise in the Mediterranean Sea on the German research vessel MARIA S. MERIAN (MSM72) in March 2018. The main objective of the cruise was to contribute to the understanding of long-term changes and trends in physical and biogeochemical parameters, such as the anthropogenic carbon uptake and to further assess the hydrographical situation after the major climatological shifts in the eastern and western part of the basin, known as the Eastern and Western Mediterranean Transients. During the cruise, multidisciplinary measurements were conducted on a predominantly zonal section throughout the Mediterranean Sea, contributing to the global GO-SHIP repeating hydrography program and adhering to the GO-SHIP requirements.