Axion-like particles (ALPs), as an extension of the standard model, define a generic class of light pseudo-scalars with a rich phenomenology because of their possible coupling to photons. Here we explore a so-far neglected opportunity to search for ALPs-photon coupling in the disappearance channel, i.e. a characteristic energy dependent suppression of gamma-ray source spectra. To verify this phenomenon, we use seven years of Fermi-LAT data of gamma-ray pulsar sources along with Fermi bubbles and investigate the presence of spectral features in accordance with photon-ALPs coupling using specific models of the large-scale Galactic magnetic field. We estimate best-fit values of parameters, i.e. photon-ALPs coupling and ALPs mass.