1 kHz fixed-target serial crystallography using a multilayer monochromator and an integrating pixel detector

Link:
Autor/in:
Erscheinungsjahr:
2019
Medientyp:
Text
Schlagworte:
  • pink beams
  • protein crystallography
  • protein structure
  • serial crystallography
  • structure determination
  • synchrotron radiation
Beschreibung:
  • Reliable sample delivery and efficient use of limited beam time have remained bottlenecks for serial crystallography (SX). Using a high-intensity polychromatic X-ray beam in combination with a newly developed charge-integrating JUNGFRAU detector, we have applied the method of fixed-target SX to collect data at a rate of 1 kHz at a synchrotron-radiation facility. According to our data analysis for the given experimental conditions, only about 3 000 diffraction patterns are required for a high-quality diffraction dataset. With indexing rates of up to 25%, recording of such a dataset takes less than 30 s.
Lizenz:
  • info:eu-repo/semantics/openAccess
Quellsystem:
Forschungsinformationssystem der UHH

Interne Metadaten
Quelldatensatz
oai:www.edit.fis.uni-hamburg.de:publications/37fabcd1-7b56-4236-b6d5-a240d81a86d0