A METHOD TO DETERMINE THE THROUGHPUT SPEED OF A PORE

Method (100) to determine the throughput speed v of at least one pore (1), comprising: • feeding (110), by a driving force F, a filiform calibration element (2) through the pore (1), said calibration element (2) having a plurality of markers (21) spaced apart by known distances (22) and configured to produce an interaction event (3) that transmits a signal away from the pore (1) upon interaction with the pore (1); • detecting (120) a plurality of interaction events (3); and determining (130) a time interval Δt between successive interaction events (3), and/or a frequency ω of interaction events (3). Filiform calibration element (2) with light sources that absorb excitation light of wavelength λ1 and to emit light of wavelength λ2 in response as markers (21). Liquid solution (20) as a tool for determining the throughput speed v, comprising a solvent (24) and a plurality of filiform calibration elements (2).