A preliminary list of the so far identified lichens collected during the 12th OPTIMA Iter toTunisia in 2014 is presented. Altogether 37 collecting sites in the northern part of the country were visited. The lichen collection comprises ≤ 300 capsules kept in SAV, of which duplicatesare being prepared for the Palermo Herbarium (PAL). Ecological and distributional data areprovided for 46 taxa, 23 of which had not been included in the published checklist of lichens of Tunisia (e. g. Anema nummularium, Athallia skii, Caloplaca pollinii, Heppia adglutinata,Scytinium palmatum, Leprocaulon microscopicum, Solenopsora grisea, and S. olivaceasubsp.olivacea). In an additional list, samples recently collected in the southern and easternMediterranean region are mentioned, which include elements characteristic of the habitats of N African mountains (e.g. the Atlas ranges) and Israel (e.g. Gloeoheppia turgida, Lichinella algerica, and Peccania fontqueriana).