Superposition of two tRNASer acceptor stem crystal structures: Comparison of structure, ligands and hydration

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Autor/in:
Erscheinungsjahr:
2010
Medientyp:
Text
Schlagworte:
  • Amino Acyl-tRNA Synthetases
  • RNA
  • Transfer
  • Genetic code
  • Ribosomes
  • Proteins
  • tRNA acceptor stem microhelix
  • RNA hydration
  • tRNA function
  • Magnesium binding sites
  • tRNASer/seryl-tRNA-synthetase
  • Superposition
  • Comparative X-ray structure analysis
  • Amino Acyl-tRNA Synthetases
  • RNA
  • Transfer
  • Genetic code
  • Ribosomes
  • Proteins
Beschreibung:
  • We solved the X-ray structures of two Escherichia coli tRNASer acceptor stem microhelices. As both tRNAs are aminoacylated by the same seryl-tRNA-synthetase, we performed a comparative structure analysis of both duplexes to investigate the helical conformation, the hydration patterns and magnesium binding sites. It is well accepted, that the hydration of RNA plays an important role in RNA-protein interactions and that the extensive solvent content of the minor groove has a special function in RNA. The detailed comparison of both tRNASer microhelices provides insights into the structural arrangement of the isoacceptor tRNA aminoacyl stems with respect to the surrounding water molecules and may eventually help us to understand their biological function at atomic resolution. © 2010 Elsevier Inc. All rights reserved.
Lizenz:
  • info:eu-repo/semantics/restrictedAccess
Quellsystem:
Forschungsinformationssystem der UHH
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Quelldatensatz
oai:www.edit.fis.uni-hamburg.de:publications/c87daf8c-3107-4869-8e2e-a8be2a7c488c