Lysophosphatidylcholine-mediated functional inactivation of syndecan-4 results in decreased adhesion and motility of dendritic cells.

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Autor/in:
Erscheinungsjahr:
2010
Medientyp:
Text
Schlagworte:
  • Humans
  • Cells, Cultured
  • Cell Movement
  • Membrane Proteins metabolism
  • Apoptosis
  • Dendritic Cells immunology
  • Phosphorylation
  • Antigens, CD86 metabolism
  • Autoimmunity
  • Cell Adhesion
  • Cell Surface Extensions metabolism
  • Fibronectins metabolism
  • HLA-DR Antigens metabolism
  • Intracellular Signaling Peptides and Proteins metabolism
  • Lysophosphatidylcholines metabolism
  • Microscopy, Video
  • Protein Kinase C-alpha metabolism
  • Protein Kinase C-delta metabolism
  • Syndecan-4 metabolism
  • Humans
  • Cells, Cultured
  • Cell Movement
  • Membrane Proteins metabolism
  • Apoptosis
  • Dendritic Cells immunology
  • Phosphorylation
  • Antigens, CD86 metabolism
  • Autoimmunity
  • Cell Adhesion
  • Cell Surface Extensions metabolism
  • Fibronectins metabolism
  • HLA-DR Antigens metabolism
  • Intracellular Signaling Peptides and Proteins metabolism
  • Lysophosphatidylcholines metabolism
  • Microscopy, Video
  • Protein Kinase C-alpha metabolism
  • Protein Kinase C-delta metabolism
  • Syndecan-4 metabolism
Beschreibung:
  • Following antigen contact, maturation and migration of DCs into lymphatic tissues are crucial to the developing immune response or maintenance of tolerance. Lysophosphatidylcholine (LysoPC) is generated during apoptosis of cells and acts as a "find-and-eat-me" signal thought to prevent autoimmunity. Moreover, LysoPC can activate PKC and initiates a signaling cascade that leads to phosphorylation and inactivation of syndecan-4 (SDC4), a heparansulfate proteoglycan integrin co-receptor. In human monocyte-derived DCs, we recently demonstrated that SDC4 is upregulated during maturation thereby stimulating DC motility. Here, we investigate the effects of LysoPC on DC motility as well as on the involvement of PKC phosphorylation-dependent regulation of DC motility by SDC4 and PKC . Employing a static adhesion assay and videomicroscopy, we show that LysoPC inhibits adhesion of DCs to fibronectin and motility of DCs by decreasing podosome formation. Moreover, DC podosome formation and motility, which both are regulated by SDC4 and subject to control by PKC -dependent phosphorylation of SDC4, were inhibited in LysoPC-matured DCs. Thus, these DC are defective in adhesion and migration. Based on our results, we hypothesize that LysoPC released during apoptosis might delay DC migration to lymphoid organs and thus prevent autoimmunity.
  • Following antigen contact, maturation and migration of DCs into lymphatic tissues are crucial to the developing immune response or maintenance of tolerance. Lysophosphatidylcholine (LysoPC) is generated during apoptosis of cells and acts as a "find-and-eat-me" signal thought to prevent autoimmunity. Moreover, LysoPC can activate PKC and initiates a signaling cascade that leads to phosphorylation and inactivation of syndecan-4 (SDC4), a heparansulfate proteoglycan integrin co-receptor. In human monocyte-derived DCs, we recently demonstrated that SDC4 is upregulated during maturation thereby stimulating DC motility. Here, we investigate the effects of LysoPC on DC motility as well as on the involvement of PKC phosphorylation-dependent regulation of DC motility by SDC4 and PKC . Employing a static adhesion assay and videomicroscopy, we show that LysoPC inhibits adhesion of DCs to fibronectin and motility of DCs by decreasing podosome formation. Moreover, DC podosome formation and motility, which both are regulated by SDC4 and subject to control by PKC -dependent phosphorylation of SDC4, were inhibited in LysoPC-matured DCs. Thus, these DC are defective in adhesion and migration. Based on our results, we hypothesize that LysoPC released during apoptosis might delay DC migration to lymphoid organs and thus prevent autoimmunity.
Lizenz:
  • info:eu-repo/semantics/restrictedAccess
Quellsystem:
Forschungsinformationssystem des UKE

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oai:pure.atira.dk:publications/6a183aa9-3c69-4773-906c-2e5c0cdc9de7