In this work, we investigate the suitability of MPI as a volumetry tool for the determination of retrograde voiding cystography of the bladder. Measurements were performed in two different experimental coil settings: first with a novel gradiometer providing three orthogonal channels and second a single-channel gradiometer at varying gradient strengths. The volumes were calculated using two different approaches: a calibration approach based on the amount of SPIOs and a threshold approach. We show that with both approaches MPI volumetry is feasible. Finally we present initial in vivo results of a retrograde voiding cystography in rats.