Stimulation of Local Cytosolic Calcium Release by Photothermal Heating for Studying Intra- and Intercellular Calcium Waves
- Link:
- Autor/in:
- Erscheinungsjahr:
- 2021
- Medientyp:
- Text
- Beschreibung:
-
A methodology is described that allows for localized Ca2+ release by photoexcitation. For this, cells are loaded with polymer capsules with integrated plasmonic nanoparticles, which reside in endo-lysosomes. The micrometer-sized capsules can be individually excited by near-infrared light from a light pointer, causing photothermal heating, upon which there is a rise in the free cytosolic Ca2+ concentration ([Ca2+ ]i ). The [Ca2+ ]i can be analyzed with a Ca2+ indicator fluorophore. In this way, it is possible to excite local lysosomal Ca2+ release in a desired target cell.
- Lizenz:
-
- info:eu-repo/semantics/openAccess
- Quellsystem:
- Forschungsinformationssystem des UKE
Interne Metadaten
- Quelldatensatz
- oai:pure.atira.dk:publications/76bb30fd-9622-4e11-836f-3356b6e35f5a